We are looking for a Research Technician to support our research studies exploring how a ubiquitin ligase contributes to DNA replication stress and repair. The position is funded by an Academy of Medical Sciences grant awarded to Dr. Panagiotis Kotsantis, and is available for 24 months.
This role involves supporting studies in DNA replication stress response signalling, including:
Generate and characterise engineered cell lines with inducible gene expression, gene depletion, and targeted genetic modifications, including domain deletions and site-directed mutations.
Investigate cellular responses to DNA damage and replication stress using a range of molecular and cell biology approaches, including immunofluorescence microscopy, immunoblotting, DNA fibre assays, and micronucleus analysis.
Examine protein localisation and chromatin-associated dynamics at sites of DNA replication and genome maintenance using advanced imaging and proteomic techniques.
Characterise protein–protein and protein–nucleic acid interactions through biochemical and proximity-based assays, including immunoprecipitation and proximity ligation assays.
Identify and analyse interaction networks and candidate substrates of a target pathway regulator using mass spectrometry-based proteomics.
Role responsibilities
As part of a friendly and collaborative team, you will be responsible for:
Preparing and conducting experiments including engineered cell line generation, DNA damage response analysis, protein interaction and localisation studies, and proteomic identification of molecular interaction networks.
Data acquisition and management, and contributing to analysis and reporting
Supporting day-to-day lab operations, including equipment maintenance, ordering supplies, record keeping, and assisting with regulatory documentation
Providing technical training to students and other lab members
About you
We are keen to hear from candidates who have:
Bachelor’s degree in biomedicine, biomedical sciences, biochemistry or a related discipline.
Experience of supporting biomedical related studies, including responsibilities such as mammalian cell culture, immunoblotting, immunofluorescence, DNA cloning, molecular biology techniques and data analysis using GraphPad-Prism and ImageJ.
Strong organisational and administrative skills, including inventory management, maintaining accurate records, and report writing.
A proactive approach to problem-solving and technical troubleshooting, with the confidence to take initiative and work independently
Excellent written and verbal communication skills, and a collaborative, team-oriented approach
Experience with the DNA fibre assay is desirable but not essential. Training will be provided for the right candidate. Most importantly, you will bring motivation, attention to detail, and enthusiasm for contributing to meaningful interdisciplinary research.
Why work with us
You will join the Genome Stability and DNA Repair Lab, led by Dr. Panagiotis Kotsantis in the Division of Biomedical and Life Sciences, and work in an interdisciplinary team with collaborators from BLS and Erasmus University (Netherlands). This role therefore offers a fantastic opportunity to gain hands-on experience across diverse research methods and environments.
The Division of Biomedical and Life Sciences, part of the Faculty of Health and Medicine, is a vibrant and interdisciplinary research environment focused on human health and disease. It offers state-of-the-art facilities and a collaborative, inclusive culture that supports both independent and team-based research, while promoting work-life balance and individual support. Find out about more about our employee benefits and life at Lancaster on our website: https://www.lancaster.ac.uk/jobs
The Faculty has held a Silver Athena SWAN award since 2014 in recognition of its good employment practice undertaken to address gender equality in higher education and research.
This position is not eligible for VISA Sponsorship.
Informal enquiries are welcome. Please contact Dr Panagiotis Kotsantis at:
[email protected]