The Pirbright Institute
The Pirbright Institute delivers world-leading research to understand, predict, detect and respond to viral disease outbreaks. We study viruses of livestock that are endemic and exotic to the UK, including zoonotic viruses, by using the most advanced tools and technologies to understand host-pathogen interactions in animals and arthropod vectors. Our major strength in understanding the immune systems of livestock provides a platform to control current diseases and respond to emerging threats. This important research would not be possible without the proportionate use of animals, which are integral to the development of new disease control measures such as vaccines and diagnostics. We have an establishment-wide commitment to the 3Rs (replacement, reduction and refinement), high standards of animal welfare, a culture of care and openness.
Our Institute is made up of a dynamic and vibrant community of staff covering a diverse set of chosen fields, backgrounds and experience. We all play a crucial role in furthering the Institute’s aspirations, advancing the frontiers of science and knowledge through our excellent scientific research, knowledge sharing and contribution; with a real focus in improving the world in which we live. Our outlook is always balanced by our strong sense of purpose, values and behaviours and an unwavering commitment to a ‘one Institute’ approach.
Organisation position and purpose
The post-holder will be a pivotal member of the Mucosal Immunology group and will support activities funded by the Institute Strategic Grants awarded by the BBSRC, Gates Foundation and FluLab.
The aim of this program is to develop broadly cross-reactive vaccines that will protect humans against influenza, including in the event of a pandemic. We will produce viral-vectored vaccines expressing NP+M1 with NA and evaluate their protective efficacy using different routes of immunization in influenza-exposed pigs. This model is designed to mimic human pre-exposure to influenza virus.
We will conduct a detailed analysis of T and B cell responses across various tissues to assess the impact of vaccination routes and identify potential correlates of protection. Pigs are an excellent model for human influenza due to their genetic, immunological, physiological, and anatomical similarities to humans. The project requires a scientist trained in a broad range of immunological and virological techniques, including FACS analysis, ELISA, ELISPOT, microneutralization, virus propagation, and plaque assays. Additionally, as part of other Gates and FluLab-funded projects, the postholder will participate in studies evaluating the ability of therapeutics and glycols to block transmission.
This project represents an exciting opportunity to be part of a consortium comprising the Pandemic Sciences Insitute, Univeristy of Oxford, the Francis Crick Institute, the Gates Foundation and the Pirbright Institutes carrying out One Health research. It is suitable for virologists and/or immunologists with a keen interest in translational research for human and animal health. The post-holder will report to the Mucosal Immunology Group Leader.
Main duties and responsibility
- Preparation of virus challenge material and quantification of virus load in tissues.
- Mammalian expression of protein antigens.
- Analysis of T cell responses after influenza infection and/or immunisation (ELISPOT, Flow cytometry).
- Assay of antibody responses (microneutralisation, ELISA, HAI, epitope mapping, ADCC).
- Contribute to animal studies to evaluate properties of transmission blockers.
Responsible for working as part of a research team, which will include
- Communicating progress and data, including presentations at internal and external meetings
- Preparation of manuscripts for peer-review, stage-based milestone reviews and end of programme assessments
- Developing and maintaining documentation associated with the health, safety and biosecurity of the research to preserve a safe working environment.
- Assist with lab management (ordering, looking after equipment, sample inventories)
- Perform outreach duties.
This job description is only an outline of the tasks, responsibilities and outcomes required of the role. The jobholder will carry out any other duties as may be reasonably required by his/her line manager. The job description and personal specification may be reviewed on an ongoing basis in accordance with the changing needs of The Pirbright Institute.
Specific requirements
Essential
- Relevant Life science degree as a minimum (MSc, Master or higher) in Molecular Biology, Virology, Immunology or other science related field
- Evidence of good oral and written communication skills including the ability to communicate effectively within a team
- Previous experience in immunology and/or virology research
- Must be able to give meticulous attention to detail including the management of samples and recording of data
Desirable
- Experience of flow cytometry and other immunological or molecular biology techniques
- Experience of working in a laboratory environment
- Evidence of networking skills with the ability to build effective collaborative relationships
- Computer literate with a good knowledge of different computer programs and experience in data presentation
Due to the nature of the work carried out by Pirbright, all successful applicants will be required to go through a comprehensive screening process including reference and qualification checks, financial and security clearances.
What we offer
- Generous Group Personal Pension Scheme and life cover (subject to age and earnings)
- 25 days annual leave per annum, pro rata, plus 10.5 public and privilege days
- Buy and sell annual leave
- Option to carry over up to two weeks annual leave
- Pirbright gym with subsidised membership
- Pirbright social club
- Pirbright Lifestyle Hub (employee discount site)
- Cycle to work
- Subsidised staff restaurant
- Childcare allowance
- Free onsite parking
- Electric Car charging facilities
