Project overview
We are looking for a Research Technician to support our research studies exploring how a ubiquitin ligase contributes to DNA replication stress repair. The position is funded by an Academy of Medical Sciences grant awarded to Dr Panagiotis Kotsantis and is available for 24 months (full-time at 1.0 FTE).
This role involves supporting studies in DNA replication stress response signalling, including:
Generate and characterise engineered cell lines with inducible gene expression/depletion, or genetic modifications.
Investigate DNA replication stress response using molecular and cell biology approaches, including immunofluorescence microscopy, immunoblotting and DNA fibres.
Examine protein localisation at sites of DNA replication and DNA damage using advanced imaging and proteomic techniques.
Characterise protein–protein and protein-DNA interactions through biochemical assays, (immunoprecipitation, PLA).
Identify and characterise substrates of a target protein using mass spectrometry-based proteomics.
Role responsibilities
You will be responsible for:
Preparing and conducting experiments including engineered cell line generation, DNA damage response analysis, protein interaction and localisation studies, and proteomic identification of molecular interaction networks.
Data acquisition/management, and contributing to analysis and reporting.
Supporting day-to-day lab operations, including equipment maintenance, ordering supplies, record keeping, and assisting with regulatory documentation.
Providing technical training to students and other lab members.
About you
We are keen to hear from candidates who have:
Bachelor’s degree in biomedicine, biomedical sciences, biochemistry or a related discipline.
Experience supporting biomedical related studies, including mammalian cell culture, immunoblotting, immunofluorescence, DNA cloning, molecular biology techniques and data analysis (GraphPad-Prism, ImageJ).
Strong organisational and administrative skills, including inventory management, maintaining accurate records, and report writing.
A proactive approach to problem-solving and technical troubleshooting, with the confidence to take initiative and work independently.
Excellent written and verbal communication skills, and a collaborative, team-oriented approach.
Experience with the DNA fibre assay is desirable but not essential. Training will be provided.
About us
You will join the Genome Stability and DNA Repair Lab, led by Dr. Panagiotis Kotsantis in the Division of Biomedical and Life Sciences (BLS). This role offers an excellent opportunity to gain hands-on experience across diverse research methods and environments.
BLS is part of the Faculty of Health and Medicine and is a vibrant and interdisciplinary research environment focused on human health and disease. It offers state-of-the-art facilities and a collaborative, inclusive culture that supports both independent and team-based research, while promoting work-life balance and individual support. Find out more here: https://www.lancaster.ac.uk/biomedical-and-life-sciences/
The Faculty has held a Silver Athena SWAN award since 2014 recognising its commitment to advancing gender equality in higher education and research.
Eligibility: This position is not eligible for VISA sponsorship.
For informal enquiries please contact Dr Panagiotis Kotsantis (
[email protected]).
